Ro5-4864 is a widely studied compound in the field of biochemical and neuropharmacological research. Its primary value lies in its high affinity for the translocator protein (TSPO), formerly known as the peripheral benzodiazepine receptor (PBR), which plays a critical role in mitochondrial function and cell regulation. Unlike traditional benzodiazepines that modulate central nervous system (CNS) activity, Ro5-4864 operates on peripheral pathways and is often used in studies focusing on inflammation, neurodegeneration, cancer, and steroid biosynthesis.

• Neuroinflammation Research
  Ro5-4864 is used to map and quantify neuroinflammation, as it binds to TSPO, which is upregulated in activated microglia—a hallmark of inflammation in neurodegenerative diseases such as Alzheimer’s and multiple sclerosis.

• Mitochondrial Function Studies
  Researchers use Ro5-4864 to investigate mitochondrial permeability transition and apoptosis. Its interaction with TSPO in the mitochondrial membrane makes it a valuable tool in understanding cell survival and death mechanisms.

• Cancer Cell Proliferation Models
  TSPO is often overexpressed in tumor cells. Ro5-4864 enables researchers to study tumor growth, progression, and the role of mitochondrial signaling in cancer biology.

• Steroidogenesis Investigation
  TSPO plays a central role in cholesterol transport into mitochondria, a precursor step in steroid hormone production. Ro5-4864 is instrumental in studies of adrenal function and stress hormone synthesis.

• PET Imaging Ligand Development
  Ro5-4864 is used in the development of radiolabeled ligands for PET (Positron Emission Tomography), allowing researchers to non-invasively visualize TSPO distribution in living organisms.

• Ischemia and Brain Injury Studies
  It helps map TSPO expression following stroke or traumatic brain injury, assisting in early diagnosis and therapeutic evaluation in animal models.

Although Ro5-4864 is structurally related to the benzodiazepine class, its pharmacological profile is significantly different. Unlike classical benzodiazepines such as diazepam or lorazepam, which exert their effects on central GABA-A receptors, Ro5-4864 selectively binds to TSPO located on mitochondrial membranes, primarily outside the CNS.

• Different Receptor Target
  Traditional benzodiazepines modulate GABA-A receptors to produce sedative, anxiolytic, or hypnotic effects. Ro5-4864, however, targets TSPO and does not affect GABAergic transmission.

• No Psychoactive Effects
  Ro5-4864 lacks the sedative, muscle relaxant, or anticonvulsant properties typically associated with CNS-active benzodiazepines. This makes it unsuitable for clinical or recreational psychoactive use.

• Peripheral Activity Only
  Ro5-4864 primarily operates in peripheral tissues and within mitochondria, making it more applicable to immunological, cardiovascular, and metabolic research rather than behavioral studies.

• Non-Addictive Profile
  Because it does not act on CNS pathways, Ro5-4864 shows no addictive or habit-forming potential, eliminating many of the concerns associated with classical benzodiazepine misuse.

Ro5-4864 stands out from other benzodiazepine derivatives due to its peripheral and mitochondrial mode of action. It is unique not only in its binding site but also in its scientific applications, which diverge completely from the tranquilizing or anxiolytic uses of CNS-active benzos.

• High Selectivity for TSPO
  Ro5-4864 binds selectively to the translocator protein, with little to no cross-reactivity with central GABA receptors, providing precise utility in mitochondrial and immune-related studies.

• Mitochondrial Modulation
  This compound has a direct influence on mitochondrial function—such as calcium signaling, reactive oxygen species (ROS) generation, and membrane potential regulation—making it valuable in apoptosis and metabolism research.

• Broad Research Applications
  Ro5-4864 is used in studies ranging from inflammation, stress responses, and neurotrauma to oncology and cardiology, setting it apart from traditional benzodiazepines that are typically limited to CNS-focused studies.

• Radioligand Versatility
  Due to its stable structure and high TSPO binding affinity, Ro5-4864 is a gold standard in the synthesis of PET radioligands for visualizing inflammatory responses in living subjects.

Proper storage of Ro5-4864 is essential to preserve its integrity, potency, and analytical reliability over time. Due to its sensitivity to environmental conditions such as heat, light, and moisture, researchers must follow strict storage protocols.

• Temperature Control
  Ro5-4864 should be stored at -20°C in a laboratory-grade freezer to maintain its stability, particularly if not used immediately after receipt.

• Light Protection
  The compound should be kept in an amber or opaque vial, as exposure to light can degrade the molecule and compromise its effectiveness in binding assays or synthesis processes.

• Moisture Avoidance
  As a fine crystalline powder, Ro5-4864 is hygroscopic and should be protected from moisture. Containers should be sealed tightly with desiccants inside to prevent humidity absorption.

• Avoid Repeated Freeze-Thaw Cycles
  Frequent temperature fluctuations can compromise chemical stability. If aliquoting is needed, it’s best to divide into small volumes for one-time use.

• Use Airtight Containers
  Vials with PTFE-lined caps are ideal to prevent oxidation and external contamination.

At RC Plug, we supply ≥98% pure Ro5-4864, verified through state-of-the-art analytical methods. This ensures our compound meets the highest standards demanded by both academic and industrial research laboratories.

• High-Performance Liquid Chromatography (HPLC)
  We use HPLC to confirm purity levels and ensure minimal presence of by-products or impurities in each batch.

• Nuclear Magnetic Resonance (NMR) Spectroscopy
  NMR validation confirms the structural integrity of Ro5-4864, ensuring it matches the expected IUPAC standard without unwanted chemical shifts or degradation.

• Mass Spectrometry (MS)
  MS analysis supports both purity and molecular weight confirmation, eliminating the possibility of contaminants or misidentified compounds.

• Certificate of Analysis (COA)
  A detailed COA accompanies each batch and is available to all customers upon request, detailing the precise purity percentage, analytical method used, and batch identifiers.

• Batch Consistency Guarantee
  We enforce strict quality control protocols to ensure that all subsequent orders meet the same purity standards for replicable research outcomes.

• Free of Controlled Substances
  Our Ro5-4864 contains no GABAergic additives or controlled CNS substances, and is lab-verified as a pure peripheral compound.

Ro5-4864 is legal to purchase in most jurisdictions when used exclusively for scientific research purposes. It is not classified as a controlled substance in many countries, but researchers and institutions must always ensure compliance with local regulatory frameworks.

• Unscheduled in Many Regions
  Ro5-4864 is not listed under the UN Convention on Psychotropic Substances or most national controlled substances acts, making it legally accessible for research in many countries.

• Restricted to Laboratory Use
  Although legal to purchase, it is explicitly restricted to in vitro or preclinical research applications. It must not be used for human or veterinary treatment.

• Institutional Responsibility
  Universities, private labs, and research organizations are responsible for ensuring that their procurement and use of Ro5-4864 align with internal and national compliance protocols.

• Export/Import Considerations
  While RC Plug provides global shipping, importation must conform to the destination country’s laws regarding chemical research materials. Customs may require a declaration of intended use.

• Documentation Support
  We offer Certificates of Analysis (COA) and Material Safety Data Sheets (MSDS) upon request to aid in regulatory reporting or laboratory inventory tracking.

• Purchase by Qualified Individuals Only
  To ensure ethical use, we sell Ro5-4864 only to verified researchers, institutions, and laboratories, not to individuals seeking unsanctioned uses.

Solubility is critical in determining how Ro5-4864 should be prepared for assays or experimental use. Ro5-4864 is hydrophobic and exhibits limited solubility in aqueous solutions but performs well in several organic solvents commonly used in pharmacological and biochemical research.

• DMSO (Dimethyl Sulfoxide)
  Ro5-4864 dissolves efficiently in DMSO, making it ideal for cell-based assays or enzyme studies. DMSO ensures uniform dispersion at molecular levels.

• Ethanol
  Ethanol is another solvent compatible with Ro5-4864 and is often used when preparing solutions for receptor-binding or imaging studies.

• Methanol (less common)
  Methanol can dissolve Ro5-4864 but may be less stable for long-term storage. It’s typically used for rapid prep in chromatography.

• Low Water Solubility
  Ro5-4864 does not dissolve effectively in water or saline, limiting its use in purely aqueous experimental systems. Formulations should include a co-solvent or carrier.

• Solubility Optimization for In Vitro Use
  Researchers often combine DMSO with culture media for bioassays, keeping final solvent concentrations low to maintain cell viability.

• Filtration and Sterility
  Once dissolved, solutions can be filtered (0.22 μm) to ensure sterility for sensitive biological applications.

Ro5-4864’s utility spans multiple disciplines due to its specificity for the translocator protein (TSPO). It is widely used in neuroscience, oncology, immunology, and molecular biology to explore mitochondrial dynamics and inflammation pathways.

• TSPO Imaging and Radiolabeling
  Ro5-4864 is a lead molecule in developing PET tracers that bind to TSPO, enabling real-time imaging of brain injury, inflammation, and cancer.

• Neurodegenerative Disease Models
  It is extensively used in Alzheimer’s, Parkinson’s, and multiple sclerosis research to evaluate glial activation and neuroinflammatory markers.

• Apoptosis and Cell Death Mechanisms
  Ro5-4864 helps examine how cells regulate survival and death through mitochondrial membrane permeability and oxidative stress signaling.

• Steroidogenesis Pathway Analysis
  In endocrine research, it supports the study of cholesterol transport and hormone biosynthesis via TSPO in adrenal and reproductive tissues.

• Cancer Research
  Many tumors overexpress TSPO. Ro5-4864 allows researchers to study its role in tumor growth, angiogenesis, and immune evasion.

• Inflammation and Immune Studies
  TSPO expression is upregulated in activated immune cells, and Ro5-4864 is used to measure immune responses in infections or autoimmune conditions.

Ro5-4864 is not psychoactive and does not exhibit the sedative, anxiolytic, or euphoric properties associated with CNS-acting benzodiazepines. This makes it safe and appropriate for strictly scientific research settings.

• No GABA-A Receptor Interaction
  Unlike classic benzos, Ro5-4864 does not modulate GABAergic neurotransmission and therefore has no CNS depressant or relaxing effects.

• No Central Nervous System Effects
  Ro5-4864 does not cross the blood-brain barrier effectively at doses used in peripheral studies, resulting in negligible CNS activity.

• No Recreational Potential
  The compound has no history or record of recreational use due to its lack of subjective effects and is not classified under drug abuse laws.

• Safe for Laboratory Handling
  Researchers do not need to take CNS-specific safety precautions such as sedation risk mitigation when working with Ro5-4864.

At RC Plug, we prioritize fast, secure, and discreet delivery of Ro5-4864 to ensure research projects stay on track. Delivery times vary depending on the destination but are consistently among the fastest in the industry.

• Domestic Orders (U.S. & EU)
  Shipments to most domestic locations arrive within 1–3 business days, with overnight delivery available in select regions.

• International Shipping
  International orders typically take 3–10 business days, depending on customs clearance and regional courier performance.

• Same-Day Dispatch
  Orders placed before our daily cutoff time are dispatched the same day, ensuring faster delivery turnaround.

• Tracking Provided
  Every shipment includes a real-time tracking number so you can monitor your package from dispatch to delivery.

• Discreet and Secure Packaging
  Packages are unlabeled and sealed in moisture-resistant, tamper-evident materials to protect both the product and your privacy.

• Refund or Reship Guarantee
  If delivery is lost or significantly delayed, we offer a full refund or reshipment as part of our 30-day customer satisfaction policy.

Yes, we provide comprehensive documentation with every order of Ro5-4864 to support regulatory compliance, research validation, and scientific reporting. This includes chemical analysis certificates and safety information to ensure proper handling and traceability in laboratory environments.

• Certificate of Analysis (COA)
  The COA confirms the compound’s purity (typically ≥98%), chemical structure validation, batch number, and analytical methodology used—vital for academic or institutional audits.

• Material Safety Data Sheet (MSDS)
  The MSDS outlines Ro5-4864’s handling protocols, hazard classification, first-aid measures, stability profile, and proper storage conditions in compliance with safety regulations.

• Batch Consistency Reports
  Upon request, we provide inter-batch comparison reports to confirm consistency in chemical quality across multiple purchases for long-term research projects.

• Spectral Data (NMR, MS)
  Researchers can request nuclear magnetic resonance (NMR) and mass spectrometry (MS) readings to validate compound identity and molecular weight against theoretical standards.

• Shipping Compliance Statement
  For international customers, we offer a shipping compliance statement that clarifies the non-medical, research-only status of the product to aid in smooth customs processing.

• Institutional Review Support
  These documents are often necessary for IRB (Institutional Review Board) submissions or internal chemical inventory audits at universities and biotech firms.

Ro5-4864 is strictly intended for laboratory research use only and must never be administered to humans or animals. Its legal and ethical use is confined to in vitro experiments or preclinical in vivo studies conducted under strict institutional oversight.

• Not Approved for Clinical Use
  Ro5-4864 has not undergone safety or efficacy testing for therapeutic application in humans and is not approved by regulatory agencies such as the FDA or EMA.

• Toxicity Profile Unverified
  While Ro5-4864 is relatively stable in controlled lab environments, its full toxicity and pharmacokinetic profile in humans remain unknown, making any use in living organisms potentially dangerous.

• Restricted to Preclinical Studies
  Ro5-4864 may be used in animal models only under ethical approval and following institutional animal welfare guidelines. It must never be used outside of a controlled research protocol.

• Legal Consequences of Misuse
  Unauthorized use in humans may result in severe legal action, including charges related to unauthorized medical practice or violation of controlled substances law, depending on jurisdiction.

• Labeling and Packaging Reflect Use Restrictions
  Every unit is clearly labeled “Not for human consumption” and is packaged in accordance with international research chemical standards.

TSPO, or the translocator protein, is an 18-kDa protein located primarily on the outer mitochondrial membrane. It was previously known as the peripheral benzodiazepine receptor (PBR). Ro5-4864’s importance lies in its role as a selective and high-affinity ligand for TSPO, making it indispensable in studying mitochondrial function, inflammation, and cellular stress responses.

• Role in Mitochondrial Function
  TSPO regulates the transport of cholesterol into mitochondria, a critical step in steroidogenesis, and plays a broader role in maintaining mitochondrial homeostasis.

• Marker of Neuroinflammation
  TSPO expression is upregulated in activated microglia, making it a key biomarker in neurodegenerative disease research, especially Alzheimer’s, Parkinson’s, and multiple sclerosis.

• Cancer and Immune Modulation
  TSPO is often overexpressed in tumor cells and immune tissues. Ro5-4864 allows researchers to explore its influence on tumor progression, immune activation, and cell proliferation.

• Cell Death and Apoptosis
  TSPO is involved in regulating mitochondrial membrane permeability, which is crucial in the early stages of apoptosis. Ro5-4864 helps delineate these pathways in cellular stress studies.

• Imaging and Diagnostic Potential
  When radiolabeled, Ro5-4864 acts as a probe for PET imaging, helping researchers map inflammatory responses in the brain and peripheral tissues with high resolution.

• Therapeutic Target Validation
  By observing how Ro5-4864 modulates TSPO activity, researchers can evaluate the potential of TSPO as a target for new drugs addressing neuroinflammation, cancer, and metabolic disorders.

Yes, RC Plug provides secure and compliant international shipping for Ro5-4864. We take careful steps to ensure that all regulatory, safety, and privacy standards are observed so that your research materials arrive safely and without delay.

• Discreet and Tamper-Proof Packaging
  All shipments are vacuum-sealed, double-layered, and packaged without any external product identification, reducing the risk of unwanted attention or seizure.

• Customs-Friendly Documentation
  We include compliant customs declarations that reflect the compound’s non-medicinal, research-only nature, helping facilitate smoother clearance across borders.

• Regulatory-Aware Logistics
  Our logistics team stays informed of changes in chemical import policies in major research hubs (e.g., EU, UK, Canada, Australia) to minimize disruptions.

• Insurance and Tracking
  Shipments include tracking numbers, and we offer optional insurance on higher-value packages to cover against loss or damage during transit.

• High Delivery Success Rate
  With years of global shipping experience, we’ve established trusted courier partnerships that help us maintain >95% on-time international delivery success.

• Customer Support for Shipping Issues
  If customs hold or shipping delays occur, our customer service team will assist in document reissuance, clarification letters, or re-dispatch if needed.

RC Plug offers a wide variety of secure, flexible payment methods to accommodate researchers globally, while also protecting your privacy and transaction anonymity. Our payment system is streamlined and optimized for international users.

• Bitcoin (BTC)
  Ideal for fast, anonymous, and borderless payments. Bitcoin is accepted for all international orders and is processed immediately upon network confirmation.

• Bank Transfer (Wire)
  Institutional or high-volume orders can be processed via direct bank transfers. This method is secure and traceable, ideal for official or institutional buyers.

• Zelle (U.S. Customers)
  Fast and convenient for customers within the United States. Zelle payments typically confirm within minutes.

• CashApp
  A fast-growing option used widely in North America, especially for small to medium research orders.

• Western Union
  Accepted worldwide, Western Union allows in-person and digital payments in local currency—useful in regions where digital banking is limited.

• No Credit Card Payments
  For buyer security and transaction discretion, we do not accept credit card payments. This policy helps prevent financial data exposure and identity theft.

• Invoice and Payment Confirmation
  Upon successful payment, you’ll receive an automated email invoice, payment confirmation, and shipping schedule update.

Ro5-4864 is available for purchase without a license in many jurisdictions as long as it is used solely for research purposes. However, researchers must confirm local legal frameworks and institutional requirements before ordering.

• No Prescription or DEA License Required
  Ro5-4864 is not classified as a scheduled or controlled substance in most regions, which means researchers do not need a special license or prescription to purchase it.

• Research-Only Restriction
  Buyers are required to agree that the compound is being acquired for legitimate scientific investigation or laboratory use only. Human or veterinary administration is strictly prohibited.

• Institutional Purchase Considerations
  Some academic or pharmaceutical institutions may have internal policies requiring chemical authorization, even if a national license isn’t mandated.

• Customs and Import Guidelines
  While no license is typically needed, customs agencies in certain countries may require proof of research purpose, such as a university or laboratory ID or purchase order.

• Supplier Terms and Compliance
  RC Plug requires buyer verification as part of our due diligence to prevent misuse and ensure we serve only legitimate research professionals.